http://www.mitbbs.com/article_t/Military/58042031.html
发信人: lubbock12 (非老非小将), 信区: Military
标 题: 2020 年最新文章，肌肉注射抗艾滋病DNA抗体在老鼠和猴子体内有效。
发信站: BBS 未名空间站 (Fri Aug 28 14:58:59 2020, 美东)
所以我不太信季博士那个肌肉注射抗新冠DNA抗体无效，这是最新前沿科技。
In vivo delivery of synthetic DNACencoded antibodies induces broad HIV-1C
neutralizing activity
Megan C. Wise, … , Laurent M. Humeau, David B. Weiner
J Clin Invest. 2020;130(2):827-837. https://doi.org/10.1172/JCI132779
1
Inovio Pharmaceuticals, Plymouth Meeting, Pennsylvania, USA. 2
Vaccine and Immunotherapy Center, The Wistar Institute, Philadelphia,
Pennsylvania, USA. 3
Perelman School of Medicine, University of
Pennsylvania, Philadelphia, Pennsylvania, USA. 4
Duke Human Vaccine Institute and 5
Department of Surgery, Duke University School of Medicine, Durham, North
Carolina, USA.
Interventions to prevent HIV-1 infection and alternative tools in HIV cure
therapy remain
pressing goals. Recently, numerous broadly neutralizing HIV-1 monoclonal
antibodies
(bNAbs) have been developed that possess the characteristics necessary for
potential
prophylactic or therapeutic approaches. However, formulation complexities,
especially for
multiantibody deliveries, long infusion times, and production issues could
limit the use of
these bNAbs when deployed, globally affecting their potential application.
Here, we
describe an approach utilizing synthetic DNA-encoded monoclonal antibodies (
dmAbs) for
direct in vivo production of prespecified neutralizing activity. We designed
16 different
bNAbs as dmAb cassettes and studied their activity in small and large
animals. Sera from
animals administered dmAbs neutralized multiple HIV-1 isolates with activity
similar to that
of their parental recombinant mAbs. Delivery of multiple dmAbs to a single
animal led to
increased neutralization breadth. Two dmAbs, PGDM1400 and PGT121, were
advanced
into nonhuman primates for study. High peak-circulating levels (between 6
and 34 μg/ml) of
these dmAbs were measured, and the sera of all animals displayed broad
neutralizing
activity. The dmAb approach provides an important local delivery platform
for the in vivo
generation of HIV-1 bNAbs and for other infectious disease antibodies.
https://www.jci.org/articles/view/132779/pdf
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